Hb H Interference on Measurement Of HbA1c With Ion-Exchange HPLC
نویسندگان
چکیده
In this article, an interference caused by hemoglobin H (Hb H), during the measurement of hemoglobin A1c (HbA1c) with ion exchange high pressure liquid chromatography (HPLC) method, was presented in blood sample of a 20-year-old male patient. HbA1c measurement was performed with Agilent 1200 HPLC system using a commercial Recipe HbA1c ion-exchange column. Hemoglobin electrophoresis was performed with Interlab G26 agarose electrophoresis automated compact system. HbA1c level was 18.2% and HbA0 level was 81.5% with ion-exchange HPLC method. Patient's fasting serum glucose was assessed before HbA1c measurement and the result was 165 mg/dL (9.16 mmol/L). On the other hand, the result of HbA0 was 87.9%, Hb H was 10.8% and Hb A2 was 1.3% with electrophoresis. Whole blood test values were within reference ranges except MCV. MCV value was 79.6 fL. It is important to keep in mind that HbA1c level might be considered falsely high with ion-exchange HPLC method because of Hb H containing sample.
منابع مشابه
Common hemoglobin variants in southern Taiwan and their effect on the determination of HbA1c by ion-exchange high-performance liquid chromatography.
BACKGROUND Patients with hemoglobin (Hb) variants may produce false HbA1c measurement. This study aimed to detect the common Hb variants in southern Taiwan and to evaluate their effect on the determination of HbA1c. METHODS A total of 1,434 samples collected for HbA1c measurement at Kaohsiung Veterans General Hospital in southern Taiwan in March 2008 were submitted for Hb variant analysis by ...
متن کاملHemoglobin Raleigh as the cause of a falsely increased hemoglobin A1C in an automated ion-exchange HPLC method.
Irreversible glycation of the hemoglobin A0 (HbA0) beta chain leads to the production of HbA1C, which can be used to monitor long-term blood glucose control in patients with diabetes mellitus. HbA1C is less positively charged than nonglycated HbA0, and this decrease in charge is the basis of ion-exchange and electrophoretic methods that measure HbA1C. We recently identified a sample that appear...
متن کاملIdentification of the First Japanese Family Harboring a Novel Hemoglobin Variant “Perpignan”, Which Caused Low HbA1c Measurement During Diabetic Follow-Up
Hemoglobin A1c (HbA1c) is an important parameter for evaluating long-term (6-8 weeks) control of blood glucose levels in patients with diabetes mellitus. We report a Japanese diabetic case who harbored abnormal Hb and demonstrated falsely low HbA1c levels in the regular ion exchange HPLC-based assay. His abnormal β-globin chain had an amino acid replacement that corresponded to glycine to serin...
متن کاملEffect of HbE trait on measurement of HbA1c by three different methods.
Sir, HbA1c testing is mainly used for monitoring glycaemic control in patients with diabetes. However, the World Health Organization now recommends that HbA1c can be used as a diagnostic test for diabetes, provided that stringent quality assurance procedures are in place and assays are standardised to criteria aligned to the international reference values. Laboratories use many different method...
متن کاملHbG-Coushatta: an unexpected discovery during HbA1c measurement.
BACKGROUND Hemoglobin (Hb) A1c is widely used as the gold standard for blood glucose level monitoring in diabetic patients. Various methods are being used in clinical laboratories for HbA1c measurement. However, the presence of Hb variants may interfere with HbA1c measurement. METHODS The HbA1c assay was performed routinely in a 55-year old woman with type 2 diabetes and hyperlipidemia that w...
متن کامل